Hi Daren,
Nope you do not need to take rear sub-frame off to take the rear wheel out. I usually either put some wood under the main stand to left the rear wheel off the ground or use a stand my other half has made which supports the frame & lifts the rear off the ground (do NOT use a stand to do this if you have woffle boxes or exhausts underneath- I have upswept exhausts). Once the relevant parts are removed the rear wheel comes out.
Any problems just ask - I'm sure someone will guide you through it on this site.
Dawn